1. Production of transgenic embryos or transgenic mice
Transgene preparation
DNA pronuclear microinjection
Transgenic embryo or mouse identification.

2. Gene targeting
Simple Knock-out
Conditional knock-out
Vector design
Probe selection
Vector construction
Es-cell culture
Blastocyst injection
ES-cell injection only
Complete service until germline transmission

3. Clean egg transfer (Re-derivation of Mouse Lines by embryo transfer)