Flow Cytometry

 

Team :  Julie Lord, Flow Cytometry Coordinator

The flow cytometry platform offers cell analysis and sorting services to IRCM researchers as well as to members of the Montreal and surrounding scientific communities. Our goal is to promote the use of flow cytometry as a tool for multiparametric analysis and to facilitate its use for a broad range of research areas. Immunophenotyping, apoptosis, cell cycle and DNA content, functional tests (calcium flow) and cell proliferation are among the techniques commonly used in cytometry.

Instrumentation

Table top analyzers

2 analyzers BD LSR Fortessa (BD Biosciences) with 5 lasers (355 nm, 405 nm, 488 nm, 561 nm and 635 nm), 18 color detection


Cyan (Beckman Coulter) with 3 lasers (405nm, 488nm and 642nm lasers), 9 color detection


Sony SA 3800 (Sony Biosciences) with 4 lasers (405nm, 488nm, 561nm and 642nm), 10 color detection


 

Cell sorting

BD FACSAria III (BD Biosciences) (under class 1 hood) with 5 lasers (355 nm, 405 nm, 488 nm, 561 nm and 635 nm), 18 color detection


Sony FP7000 (Sony Biosciences) (under class 2 hood) with 5 lasers (349 nm, 405 nm, 488 nm, 561 nm et 637 nm), 40+ color detection


 

Services

The flow cytometry platform also offers services of data analysis, training and consultation.

Autre instrumentation

AutoMACS, automated device for cell separation using magnetic beads


Extracellular flow analyzer XF24-3 (Seahorse Bioscience), a fully integrated instrument that simultaneously measures the two major energy pathways – aerobic respiration and glycolysis – in a convenient microplate format


ADVIA 120, a hematological analyzer on human and animal whole blood


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Flow Cytometry services

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